Journal: Pediatrics and neonatology

Article Title: The significance of serum and fecal levels of interleukin-6 and interleukin-8 in hospitalized children with acute rotavirus and norovirus gastroenteritis.

doi: 10.1016/j.pedneo.2013.05.008

Figure Lengend Snippet: Figure 1 Serum (A) interleukin-6 (IL-6) and (B) interleukin-8 (IL-8) levels in children with rotavirus, norovirus, bacterial gastroenteritis, and in healthy controls. The median with interquartile range (IQR) are shown in each group. p value for comparisons by Kruskal-Wallis test; *p < 0.05, by Dunn’s mul- tiple comparison test between groups.

Article Snippet: IL-6 and IL-8 levels were measured with a Quantikine Human Interleukin Immuno-assay (R&D Systems, Minneapolis, MN, USA) using the principle of quantitative sandwich enzyme immuno-assay with monoclonal antibodies specific for IL-6 and IL-8.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression

doi: 10.1371/journal.pone.0124865

Figure Lengend Snippet: (A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP1, MMP2, MMP3, MMP9, and TIMP1 in the cell culture supernatants.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, MANN-WHITNEY

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Membrane-Type Serine Protease-1/Matriptase Induces Interleukin-6 and -8 in Endothelial Cells by Activation of Protease-Activated Receptor-2

doi: 10.1161/01.atv.0000258862.61067.14

Figure Lengend Snippet: Figure 5. Increased MT-SP1/matriptase expression in human atherectomies: interaction of monocytic MT-SP1/matriptase and endothelial PAR-2 contributes to IL-8 release. A, Total RNA was prepared from human atherectomies (n26) and from undis- eased vessels (n8). Quantitative RT-PCR for MT-SP1/ matriptase was performed, and mRNA copies were determined using a standard curve of pre-quantified PCR products specific for the target mRNA. MT-SP1/matriptase copies are shown rela- tive to the GAPDH copies in the sample. Each dot represents one analyzed sample. B, Immunohistochemistry of human atherectomies using anti–MT-SP1/matriptase monoclonal anti- body M32 (10 g/mL). Nuclei were stained with Mayer hema- laun. Panels I and II (magnification 40) show selective MT-SP1/matriptase staining in atherosclerotic vessels. Arrows mark cells adhering to the endothelial layer. Panel IgG (40) shows the isotype control. C, Coincubation of HUVECs and monocytic Mono-Mac-6 (MM6) cells under static conditions at a 1:1 ratio. Cells were transfected with siPAR-2 (HUVECs) or siMT-SP1 (MM6) and 32 hours later coincubated for 16 hours. Supernatants were analyzed for IL-8 by immunoassay. Data are shown as meansSEM of 3 independent experiments, asterisks indicate P0.05 vs HUVECs.

Article Snippet: For cytokine release assays cells were seeded in 96-well plates and supernatants were analyzed using immunoassay (human IL-8, R&D Systems, Wiesbaden, Germany) or Cytometric Bead Array (CBA human Chemokine-I, Becton Dickinson, San Diego, USA).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining, Control, Transfection